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human recombinant her2 erbb2 protein  (Sino Biological)


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    Structured Review

    Sino Biological human recombinant her2 erbb2 protein
    Human Recombinant Her2 Erbb2 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+her2/Human+HER2+ErbB2+Protein/pmc13099425-283-19-23
    Average 95 stars, based on 75 article reviews
    human recombinant her2 erbb2 protein - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-life.
    Article Snippet: Harvested supernatant containing secreted receptors was further purified with a HisTrap HP column supplied with Ni2+ ions (GE Healthcare, catalog number: 17-5248-02) (90). .. Ninety-six-well ELISA plates (Costar, catalog number: 10544522) were coated with 1.0 μg/mL recombinant human HER2 (Sino Biological, catalog number: 10004-HCCH) diluted in PBS (Merck, catalog number: D8537), and incubated ON at 4 °C. .. Next, the wells were blocked for 1 h at room temperature (RT), using PBS with 4% skimmed milk (S) (ITW reagents, catalog number: A0830).

    Article Title: Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-life
    Article Snippet: Harvested supernatant containing secreted receptors was further purified with a HisTrap HP column supplied with Ni 2+ ions (GE Healthcare, catalog number: 17-5248-02) ( ). .. Ninety-six-well ELISA plates (Costar, catalog number: 10544522) were coated with 1.0 μg/mL recombinant human HER2 (Sino Biological, catalog number: 10004-HCCH) diluted in PBS (Merck, catalog number: D8537), and incubated ON at 4 °C. .. Next, the wells were blocked for 1 h at room temperature (RT), using PBS with 4% skimmed milk (S) (ITW reagents, catalog number: A0830).

    Recombinant:

    Article Title: Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-life.
    Article Snippet: Harvested supernatant containing secreted receptors was further purified with a HisTrap HP column supplied with Ni2+ ions (GE Healthcare, catalog number: 17-5248-02) (90). .. Ninety-six-well ELISA plates (Costar, catalog number: 10544522) were coated with 1.0 μg/mL recombinant human HER2 (Sino Biological, catalog number: 10004-HCCH) diluted in PBS (Merck, catalog number: D8537), and incubated ON at 4 °C. .. Next, the wells were blocked for 1 h at room temperature (RT), using PBS with 4% skimmed milk (S) (ITW reagents, catalog number: A0830).

    Article Title: Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-life
    Article Snippet: Harvested supernatant containing secreted receptors was further purified with a HisTrap HP column supplied with Ni 2+ ions (GE Healthcare, catalog number: 17-5248-02) ( ). .. Ninety-six-well ELISA plates (Costar, catalog number: 10544522) were coated with 1.0 μg/mL recombinant human HER2 (Sino Biological, catalog number: 10004-HCCH) diluted in PBS (Merck, catalog number: D8537), and incubated ON at 4 °C. .. Next, the wells were blocked for 1 h at room temperature (RT), using PBS with 4% skimmed milk (S) (ITW reagents, catalog number: A0830).

    Article Title: Neutrophil membrane-coated circular RNA nanoparticles for targeted immunotherapy in HER2-positive breast cancer brain metastasis
    Article Snippet: .. Recombinant human HER2 (Sino Biological, China) or recombinant mouse PD-1 (Sino Biological, China) was immobilized on a CM5 sensor chip (Cytiva, USA) using an amino coupling assay kit (Cytiva, USA). .. Antibodies were serially diluted to concentrations of 500, 100, 20, 4, and 0.8 nM in 1× HBS-EP + buffer and flowed over the chip at a rate of 30 μL/min using a Biacore T200 system.

    Article Title: Antibody-lectin chimeras for glyco-immune checkpoint blockade
    Article Snippet: When indicated, 10 μM ganglioside GD2 (Cayman Chemical, 25487) was added to beads for 30 min and incubated with end-over-end mixing. .. For HER2 conjugated beads, recombinant human HER2 (Sino Biological, 10004-H08H4) was added at 1000 nM. ..

    Article Title: Enhanced therapeutic potential of antibody fragment via IEDDA-mediated site-specific albumin conjugation
    Article Snippet: Immunoplates (96-well, flat, #32,396) were obtained from SPL Life Sciences (Gyeonggi, Korea). .. Recombinant human HER2 (#10,004-HCCH) was sourced from Sino Biological (Beijing, China). .. Tween-20 (#1,610,781) was obtained from Bio-Rad (Hercules, CA, USA).

    Article Title: Antibody-lectin chimeras for glyco-immune checkpoint blockade.
    Article Snippet: Genotypes were confirmed using PCR in-house, as previously described20,63, or with validated qPCR probes (Transnetyx). .. For HER2 conjugated beads, recombinant human HER2 (Sino Biological, 10004-H08H4) was added at 1000 nM. ..

    Article Title: Neutrophil membrane-coated circular RNA nanoparticles for targeted immunotherapy in HER2-positive breast cancer brain metastasis.
    Article Snippet: .. Recombinant human HER2 (Sino Biological, China) or recombinant mouse PD-1 (Sino Biological, China) was immobilized on a CM5 sensor chip (Cytiva, USA) using an amino coupling assay kit (Cytiva, USA). .. Antibodies were serially diluted to concentrations of 500, 100, 20, 4, and 0.8 nM in 1× HBS-EP + buffer and flowed over the chip at a rate of 30 μL/min using a Biacore T200 system.

    Article Title: Albubody: an engineered scFv variant platform for site-specific drug conjugation and enhanced tumor efficacy
    Article Snippet: Antibody-drug conjugates (ADCs) represent a promising approach for targeted cancer therapy.. Although single-chain variable fragments (scFvs) hold potential as drug carriers, their application in ADCs has been limited.. This study introduces Albubody, an engineered scFv fused with an albumin-binding domain (ABD), as a potential ADC scaffold designed to interact with serum albumin in vivo.

    Incubation:

    Article Title: Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-life.
    Article Snippet: Harvested supernatant containing secreted receptors was further purified with a HisTrap HP column supplied with Ni2+ ions (GE Healthcare, catalog number: 17-5248-02) (90). .. Ninety-six-well ELISA plates (Costar, catalog number: 10544522) were coated with 1.0 μg/mL recombinant human HER2 (Sino Biological, catalog number: 10004-HCCH) diluted in PBS (Merck, catalog number: D8537), and incubated ON at 4 °C. .. Next, the wells were blocked for 1 h at room temperature (RT), using PBS with 4% skimmed milk (S) (ITW reagents, catalog number: A0830).

    Article Title: Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-life
    Article Snippet: Harvested supernatant containing secreted receptors was further purified with a HisTrap HP column supplied with Ni 2+ ions (GE Healthcare, catalog number: 17-5248-02) ( ). .. Ninety-six-well ELISA plates (Costar, catalog number: 10544522) were coated with 1.0 μg/mL recombinant human HER2 (Sino Biological, catalog number: 10004-HCCH) diluted in PBS (Merck, catalog number: D8537), and incubated ON at 4 °C. .. Next, the wells were blocked for 1 h at room temperature (RT), using PBS with 4% skimmed milk (S) (ITW reagents, catalog number: A0830).



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